Open Source MATLAB Deep Learning Frameworks

Description: Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. To obtain reproducible and reliable quantitative data, we developed an easy-to-use tool named CometAnalyser. CometAnalyser is an open-source deep-learning tool designed for the analysis of both fluorescent and silver-stained wide-field microscopy images. Once the comets are segmented and classified, several intensity/morphological features are automatically exported as a spreadsheet file. Video Tutorial: CometAnalyser is written in MATLAB. It works with Windows, Macintosh, and UNIX-based systems. Please, download the sample datasets and test it watching the video tutorial to understand how it works: https://www.youtube.com/watch?v=vh2VFnMw50A Contacts: filippo.piccinini85@gmail.com beleonattila@gmail.com

To accelerate the analysis of tumors' spheroids, different deep learning networks were trained to automatize the segmentation process. The code provides the trained networks based on Vgg16, Vgg19, ResNet18, and ResNet50 ready to be used for segmentation purposes. It also provides Matlab functions ready to be used to train new networks, segment new images, and measure the quality of the training using different quantitative parameters.